#Scaffold protein h1 series
In the current study, a new series of benzimidazoimidazole, benzimidazothiazole, benzimidazotriazine, and benzimidazoquinazoline scaffolds was synthesized via C–H cycloamination, using a metal-free synthetic pathway, as potent antiproliferative antiangiogenic molecules against breast (MCF-7) and lung (A549) cancer cell lines. Stable for 1 year at 2-8☌ from date of receipt.One of the current approaches used in drug discovery and development is the synthesis of novel small compounds from existing structural motifs via molecular hybridization. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Western Blotting Analysis: 2 µg/mLof this antibody detected GEF-H1 in untreated HCT116 cells, but not in cells pretreated with MEK1/2 inhibitors PD98059 and U0126. Uncharacterized bands may be observed in some lysate(s).Įvaluated by Western Blotting in lysates from untreated HCT116 cells and those pretreated with MEK1/2 inhibitors PD98059 and U0126. Linear peptide from C-terminal region of human GEF-H1.Ĭlone 14B11 is a mouse monoclonal antibody that specifically detects human Rho guanine nucleotide exchange factor 2 (GEF-H1). Western Blotting Analysis: A representative lot detected GEF-H1 in Western Blotting applications (Cullis, J., et. MABC1097, is a mouse monoclonal antibody that detects Rho guanine nucleotide exchange factor 2 (GEF-H1) and has been tested for use in Western Blotting. Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.Īnti-GEF-H1, clone 14B11, Cat. It also has two phorbol-ester/DAG-type zinc finger (aa 587-611) and the C-terminal coiled coil domains (aa 798-867), both of which are required for association with microtubules. GEF-H1 contains a DH (DBL-homology) domain (aa 235-432) that interacts with and promotes loading of GTP on RhoA and tyrosine phosphorylation of RIPK2. It is also reported to be important for the growth and survival of HRASV12 transformed cells. GEF-H1 I known to contributes to cell survival and growth in HRASV12- transformed cells and is reported to be essential for activation of MAP kinase pathway in response to HRASV12. GEF-H1 can act as an adaptor protein that links PP2A B subunits to KSR-1 and mediate the dephosphorylation of KSR-1 serine 392 and activate MAPK signaling. GEF-H1 is involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and innate immune response. It localizes to the tips of cortical microtubules of the mitotic spindle during cell division and is released upon microtubule depolymerization. GEF-H1 is a microtubule-associated guanine nucleotide exchange factor (GEF) for the Rho family of small GTPases that activates Rho-GTPases by promoting the exchange of GDP for GTP. Rho guanine nucleotide exchange factor 2 (UniProt: Q92974 also known as Guanine nucleotide exchange factor H1, GEF-H1, Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40) is encoded by the ARHGEF2 (also known as KIAA0651, LFP40) gene (Gene ID: 9181) in human. Proliferating cell nucleolar antigen p40.Rho guanine nucleotide exchange factor 2.
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